Year Established: 2018 Start Date: 2018-03-01 End Date: 2019-02-28
Total Federal Funds: $15,000 Total Non-Federal Funds: $31,322
Principal Investigators: Greg Michalski
Abstract: The proposed research will use a combination of laboratory experiments, field sampling, and community based water sampling to assess the sources of nutrients in the Wabash River watershed and how these nutrients are utilized by potentially harmful algae and denitrifying bacteria. The novelty of the research will be the use of multiple naturally occurring isotopes in nitrate and phosphate that can be used as tracers of N/P sources and as evidence of in-stream nutrient loss processes. The laboratory experiments will consist of controlled incubations of cyanobacteria and naturally occurring algae obtained from the Wabash River and ponds, ditches, and streams that funnel into the main river. They will be grown at different temperatures and variable nutrient loading and the isotope enrichment factors for 15N in nitrate and 17O, 18O in both nitrate and phosphate will be determined. The same enrichment factors will be determine for denitrification occurring in an agricultural field bioreactor and in incubation experiments using Wabash River sediments. Determining these isotope enrichment factors is important for understanding the isotopic composition of nitrate and phosphate in the Wabash. Current hypotheses suggest that the isotopic composition of nitrate (and phosphate) in a water body reflects a mixing of different N/P sources. We propose an alternative hypothesis: That N/P loss by algal uptake and denitrification impose their own isotope signal and this can result in improper source apportionment using the existing mixing paradigm. Our preliminary data suggests that high 5N and 8O values detected in the Wabash are not evidence of combination of sewage/manure and atmospheric nitrate sources, rather may be N loss by eutrophication and/or HABs. We further hypothesize that similar changes in the 8O of phosphate would be manifest during P uptake by algae. Thus, isotopes maybe useful in understanding nutrient utilization during HAB and eutrophication events.