Water Resources Research Act Program

Details for Project ID 2016KY260B

The use of eDNA to detect bacterial genetic markers associated with fecal contamination in the Triplett Creek Watershed, Rowan County, Kentucky

Institute: Kentucky
Year Established: 2016 Start Date: 2016-03-01 End Date: 2017-02-28
Total Federal Funds: $4,836 Total Non-Federal Funds: $9,854

Principal Investigators: Geoff Gearner

Abstract: The objective of this investigation is to develop and evaluate the use of bacterial genetic targets as markers of fecal contamination in the Triplett Creek Watershed for the purpose of identifying point and host sources of fecal pollution. Specifically, this project will use quantitative polymerase chain reaction (qPCR) to evaluate DNA extracted from filtered water samples (environmental or eDNA) collected from long standing sampling sites in the Triplett Creek Watershed for the presence of genetic markers associated with fecal bacteria.