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Chapter A7. Biological Indicators

National Field Manual for the Collection of Water-Quality Data (TWRI Book 9)

7.0 Five-Day Biochemical Oxygen Demand (11/2003) [3.71 KB PDF]

By G.C. Delzer and S.W. McKenzie

This report is in pdf format. Download a copy of Adobe Acrobat Reader for free.


The presence of a sufficient concentration of dissolved oxygen is critical to maintaining the aquatic life and aesthetic quality of streams and lakes. Determinng how organic matter affects the concentration of dissolved oxygen (DO) in a stream or lake is integral to water-quality management. The decay of organic matter in water is measured as biochemical or chemical oxygen demand. This report describes the field protocols used by U.S. Geological Survey (USGS) personnel to determine the five-day test for biochemical oxygen demand.


7.0 - Five-day biochemical oxygen demand

7.0.1 - Equipment and supplies

7.0.2 - Sample collection and storage

7.0.3 - Five-day test for biochemical oxygen demand

7.0.4 - Calculations

7.0.5 - Troubleshooting

7.0.6 - Reporting

Comments/Errata page

 [Back] Return to Chapter A7 Biological Indicators Table of Contents

 [Back] Return to Field Manual Table of Contents

Citation for Section 7.0

Delzer, G.C., and McKenzie, S.W., 2003, Five-day biochemical oxygen demand: U.S. Geological Survey Techniques of Water-Resources Investigations, book 9, chap. A7, section 7.0, November, accessed __list date__ from

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