"A Supplement to--Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples (U.S. Geological Survey
Techniques of Water-Resources Investigations, Book 5, Chapter A4)"



In Reply Refer To:                               October 23, 1979
EGS-Mail Stop 412


QUALITY OF WATER BRANCH TECHNICAL MEMORANDUM NO. 80.02

Subject: PUBLICATIONS: "A Supplement to--Methods for Collection 
and Analysis of Aquatic Biological and Microbiological Samples 
(U.S. Geological Survey Techniques of Water-Resources 
Investigations, Book 5, Chapter A4)" edited by Phillip E. Greeson 
(USGS Open-File Report 79-1279, 1979, 92 pp.).

I am pleased to announce the availability of the subject 
publication, which contains several methods used by the U.S. 
Geological Survey to collect, preserve, and analyze waters for 
their biological and microbiological properties. The open-file 
report supplements the biological TWRI that was published in 1977. 
Included in the supplement are five new methods, a new section of 
selected taxonomic references for Ostracoda, and six revised 
methods. The new methods include:
     total bacteria by epifluorescence 
     Pseudomonas aeruginosa 
     identification and enumeration of periphytic diatoms 
     chlorophyll a and b in phytoplankton by high pressure liquid 
       chromatography 
     chlorophyll a and b in periphyton by high pressure liquid 
       chromatography

The revised methods, which supercede methods published in the 1977 
TWRI, include:
     inverted microscope method for phytoplankton 
     glass-fiber filter method for seston 
     gravimetric method for periphyton biomass 
     biomass/chlorophyll ratio for plankton 
     biomass/chlorophyll ratio for periphyton 
     algal growth potential

The biological TWRI currently is undergoing a complete revision, 
which should be available in about mid-l981.

The supplement is being distributed automatically to district 
offices and to those on a special WRD mailing list. Extra copies 
are available upon request to the Ouality of Water Branch (Mail 
Stop 412, National Center, Reston, Virginia 22092).



                                  R. J. Pickering 
                                  Chief, Quality of Water Branch

WRD Distribution: A, B, S, FO, PO