WATER QUALITY: Analytical Procedures - "Immature Simuliidae, Methods for Identification (Dissection and Mounting)" by S. S. Hahn and L. J. Tilley January 21, 1974 QUALITY OF WATER BRANCH TECHNICAL MEMORANDUM NO. 74.09 Subject: WATER QUALITY: Analytical Procedures - "Immature Simuliidae, Methods for Identification (Dissection and Mounting)" by S. S. Hahn and L. J. Tilley In studies using benthic invertebrates as indicators of water quality, problems arise in the identification of some taxa of the insect Family Simuliidae. Although many of the Simuliidae can be identified with little or no difficulty, some require dissection and slide mounting for microoscopic examination. Simuliidae (commonly called simuliids or blackflies, sometimes called buffalo gnats) are insects found worldwide. The larvae occur only in swifty flowing freshwaters. Their general taxonomic classification is as follows: Phylum: Arthropoda Class: Insecta Order: Diptera (flies) Family: SIMULIIDAE Genus (common): Prosimulium. The attached provisional procedure describes the anatomical features used for identification of the Simuliidae and the method for dissecting and preparing microscope mounts. Questions or comments pertaining to the procedure should be directed to the Chief, Quality of Water Branch (Mail Stop 412, Reston, Virginia). R. J. Pickering Attachment WRD Distribution: A, FO-LS, PO