State Water Resources Research Institute Program

Project ID: 2012ND258B
Title: Quantifying Estrogens Bound to Soil and Manure Colloids and Assessing Their Bioavailability
Project Type: Research
Start Date: 3/01/2012
End Date: 2/28/2013
Congressional District: 001
Focus Categories: Water Quality
Keywords: Estrogens, bioavailability, Soil and Manure colloids
Principal Investigator: Casey, Francis Xavier McKeon
Federal Funds: $ 5,000
Non-Federal Matching Funds: $ 10,000
Abstract: The natural estrogen, 17Β-Estradiol (E2), is the most potent endocrine disrupting compound. The issue of reproductive hormones in the environment is of particular relevance to animal agricultural because of the association of hormone detections with manure management practices. Natural hormone concentrations have been detected in runoff and receiving surface waters as a result of field manure application. Reproductive hormones have also been measured in subsurface waters in proximity to intensive livestock production. Widespread E2 detections throughout the soil profile and shallow groundwater in fields in and around a swine (Sus scrofa domesticus) farm has been found in western North Dakota.

The objectives of this proposed project are the following: (i) Evaluate the association of radiolabeled estradiol with both the Dissolved organic carbon (DOC) and colloidal organic carbon (COC) fraction derived from liquid swine manure, soil and liquid swine manure applied to soil (ii) Simulate rainfall events to investigate the dislodgment of radiolabeled estradiol from the COC fraction (iii) Evaluate the estrogen potency of estradiol bound to the DOC/COC, DOC and COC fraction using estrogen receptor competitive assays.

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