State Water Resources Research Institute Program

Project ID: 2011KY171B
Title: Metagenomic analysis of microbial urea transformation in soil
Project Type: Research
Start Date: 3/01/2011
End Date: 2/29/2012
Congressional District: KY 6th
Focus Categories: Nitrate Contamination, Agriculture, Non Point Pollution
Keywords: urease inhibitors, microbial processes, biotransformations
Principal Investigators: Moe, Luke; Coyne, Mark
Federal Funds: $ 5,000
Non-Federal Matching Funds: $ 10,034
Abstract: Metagenomics (defined as the genomic analysis of an assemblage of organisms) comprises a suite of culture-independent microbial techniques used for comprehensive genomic analysis of entire microbial communities. Metagenomic techniques circumvent any bias associated with culturability of bacteria, and therefore give a more representative view of microbial community structure and function. The objective of this project is to use metagenomic analysis to identify and characterize genes encoding urease enzymes from urea-amended soils. We will study two types of soils: one soil will have a history of urea application while the other will not. Among the urea-exposed soils, we will also target those soils that have had urease inhibitors applied. We expect to develop and validate the functional metagenomic screen and identify genes encoding microbial ureases from each metagenomic sample. We will begin phylogenetic analysis of the urease genes and start tests on the susceptibility of the urease enzymes to urease inhibitors.

Progress/Completion Report, 2011, PDF

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