State Water Resources Research Institute Program

Project ID: 2007NJ144B
Title: Using assimilated C-DNA to fingerprint active microorganisms in methylmercury demethylation by stable-isotope probing
Project Type: Research
Start Date: 3/01/2007
End Date: 2/29/2008
Congressional District: 6th
Focus Categories: Hydrogeochemistry, Toxic Substances, Methods
Principal Investigator: Barkay, Tamar; Yu, Riqing (Rutgers University)
Federal Funds: $ 5,000
Non-Federal Matching Funds: $ 10,980
Abstract: The project will test SIP as a method that might provide a promising and powerful approach in microbial MeHg demethylation studies. The proposed project will examine if the C1 group of CH3Hg is assimilated into nucleic acids of exposed cells and how much of the carbon is assimilated. This is important for the understanding of MeHg demethylation and for correctly calculating demethylation rates. Phylogenetic survey of metabolically active species or groups of bacteria and archaea involving in CH3Hg demethylation will suggest strategies for further enrichment isolation of specific strains, and future studies with such strains will add to knowledge that is relevant to CH3Hg bioremediation.

Progress/Completion Report, PDF

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