State Water Resources Research Institute Program


Project ID: 2006SD74B
Title: Microbial Indices of Soils and Water Associated with Vegetated Treatment Areas (VTAs) from Five Animal Feeding Operations (AFOs) in South Dakota
Project Type: Research
Start Date: 3/01/2006
End Date: 2/28/2008
Congressional District: First
Focus Categories: Water Quality, Surface Water, Acid Deposition
Keywords: vegetated treatment area; animal feeding operation; Escherichia coli; microbial
Principal Investigators: Bleakley, Bruce; Trooien, Todd P.
Federal Funds: $ 15,000
Non-Federal Matching Funds: $ 30,007
Abstract: Problem: Nutrient and sediment loads from animal feeding operations (AFOs) can negatively impact the quality of surface waters and groundwaters. One accepted way to reduce nutrient and sediment loads from AFOs is by use of basin technologies, which are effective but can be costly, and lead to air quality problems due to unpleasant odors. The potential for development and implementation of alternative non-basin technologies interests a variety of groups, including the South Dakota Cattlemen's Association, South Dakota Farm Bureau, South Dakota Association of Conservation Districts, South Dakota State University, South Dakota Department of Agriculture, Natural Resources Conservation Service, South Dakota DENR, and cattle producers. The Iowa Cattlemen's Association (ICA) is also interested.

An EPA funded grant, "Evaluating the Performance of Vegetated Treatment Areas," (Dr. Todd Trooien, P.I.), is in progress. Its goal is evaluation of the technical and financial feasibility of vegetated treatment areas (VTAs) as a non-basin alternative for reducing nutrient and sediment loads from AFOs having less than 1,000 animal units. Each of five AFOs in different areas of South Dakota have had or will have VTAs established. Performance of each VTA will be measured by sampling inflows and outflows from vegetated areas. The samples will be analyzed for nutrients (N and P), salts, sediment, and numbers of fecal coliform bacteria. Data from these measurements will allow calculation of water and salt balances, loss or gain of nutrients, removal of sediment, and fecal coliform numbers. Data will be entered in a basin model to simulate basin performance, that will be compared to measured VTA performance. Samples have been obtained during 2005 and 2006, and will continue to be obtained during 2007.

For the EPA project, only numbers of Escherichia coli other than E. coli O157:H7 are being measured. Other aspects of the microbiology of the inflow and outflow areas associated with the VTAs are not addressed, which will be the focus of this proposal.

Objectives: Differences in the microbiology of soils in the inflow and outflow areas associated with VTAs will be assayed by measuring the following microbial indices: (a) Soil respiration; (b) oxidation/reduction potential; (c) heterotrophic microbial activity; (d) soil bacterial diversity; (e) numbers of total culturable fecal coliforms (including non-pathogenic E. coli but including other culturable fecal coliforms as well); and (f) presence or absence of culturable pathogenic E. coli O157:H7. Work outlined in this proposal would obtain data on these microbial indices, and add it to the data sets from the EPA project, to get a better idea of the number and activity of microbes in soils associated with inflow and outflow areas.

Methods: Soil respiration will be measured in the field with a portable soil respirometer. Oxidation/reduction potential of wet soils will be assayed in the field with a portable meter fitted with an oxidation/reduction electrode. Heterotrophic microbial activity will be assayed in the laboratory by assaying ability of soil samples to hydrolyze fluorescein diacetate. Soil bacterial diversity will be assayed in two ways: (1) by use of Biolog EcoPlates that assay the ability of a soil microbial community to utilize 96 different carbon sources; and (2) by molecular methods, using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Numbers of total culturable fecal coliforms will be obtained by use of selective and differential culture media. Presence or absence of E. coli O157:H7 will be by serologic methodology.

Progress/Completion Report, PDF
Progress/Completion Report, 2009, PDF

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