WATER RESOURCES RESEARCH GRANT PROPOSAL
Project ID: 2004NH31B
Title: Use of Male Specific Bacteriophage as an Indicator For the Presence of Hepatitis A Virus in Alkaline Stabilized Biosolids Intended for Land Application
Project Type: Research
Focus Categories: Treatment, Methods, Toxic Substances
Keywords: Biosolids, Indicators, bacteriophage MS-2, Hepititis A
Start Date: 03/01/2004
End Date: 02/28/2005
Federal Funds: $22,945
Non-Federal Matching Funds: $72,123
Aaron B. Margolin
The routine examination of alkaline treated biosolids for the examination of viral pathogens according to the 503 regulations is a time consuming, labor intensive process which is very costly and slow to provide results, sometimes taking up to 4 weeks. An alternative organism, one which is relatively easy to assay, relatively inexpensive and rapid to provide results would act as a good surrogate for the overall monitoring of biosolids prior to their land application.
Indicator organisms are certain species of organism believed to indicate
the presence of a larger set of pathogens that may be present in a given sample.
Fecal coliforms are used as the indicator organisms in the Part 503 Rule to
classify Class A biosolids and to determine their health hazards. Fecal coliforms
are also used to indicate wastewater treatment efficiency and are measured
to determine if bacteria have repopulated when Class A biosolids are stored
before land application. We have found that there does not exist a correlation
between the presence of fecal coliforms and enteric viruses found in biosolids
(Brabants and Margolin, 2003).
We have also found that biosolids evaluated for certain enteric viruses according to the 503 Rule often go undetected (Chaprone et. al., 2002). There are several reasons for this, but predominately viruses such as rotavirus, adenovirus and hepatitis A virus could not be cultivated at the time of the development of the 503 Rule. However, since the development of the rule, there have been many advances in virology and we are now able to cultivate and detect these viruses in cell culture.
The major goal of this research is to evaluate the use of bacteriophage MS-2 as an indicator for the inactivation of Hepatitis A virus. We propose that hepatitis A will be inactivated under liming conditions more rapidly then bacteriophage MS-2.