Project ID: 2002CA3B

Title: Perchlorate Removal in Groundwater Using Immobilized Cell-Free, Purified and Recombinant Perchlorate Reductases from the Perchlorate Respiring Bacterium, Perclace

Project Type: Research

Focus Categories: Water Quality, Treatment, Toxic Substances

Keywords: groundwater, contamination, perchlorate, perchlorate reductase, enzyme purification, gene cloning, enzyme over-expression, recombinant enzyme, immobilized enzyme, packed bed bioreactor and on-site bioremediation

Start Date: 03/01/2002

End Date: 02/28/2003

Federal Funds: $20,653

Non-Federal Matching Funds: $28,100

Congressional District: 43

Principal Investigator:
William T. Frankenberger, Jr.
University of California, Riverside campus

Abstract

William T. Frankenberger is PI on this project
Dr. William T. Frankenberger
Environmental Science, Riverside campus
email: william.frankenberger@ucr.edu
phone: 909-787-2954

Dr. Frankenberger will share with 4 other PIs from California in the award distribution.

Microbial reduction of perchlorate to environmentally-acceptable innocuous end products is currently an area of intense interest. Although various microorganisms have been shown to reduce perchlorate and chlorate, to either chlorite or completely to chloride and molecular oxygen, there is limited biochemical and molecular data on the enzyme systems involved in perchlorate reduction. Such data would provide important information for the design and application of an efficient biological-based ground water remediation strategy for perchlorate. This project will evaluate perchlorate reduction by cell-free extracts of Perclace in aqueous batch experiments. Data achieved from thie experiments would be useful for field-scale studies of groundwater treatment. The use of a cell-free enzyme system for perchlorate reduction would help avert the need for a post-reactor to destroy biomass in the effluent.

Progress/Completion Report PDF