WATER RESOURCES RESEARCH GRANT PROPOSAL
Project ID: 2002CA3B
Title: Perchlorate Removal in Groundwater Using Immobilized Cell-Free, Purified and Recombinant Perchlorate Reductases from the Perchlorate Respiring Bacterium, Perclace
Project Type: Research
Focus Categories: Water Quality, Treatment, Toxic Substances
Keywords: groundwater, contamination, perchlorate, perchlorate reductase, enzyme purification, gene cloning, enzyme over-expression, recombinant enzyme, immobilized enzyme, packed bed bioreactor and on-site bioremediation
Start Date: 03/01/2002
End Date: 02/28/2003
Federal Funds: $20,653
Non-Federal Matching Funds: $28,100
Congressional District: 43
William T. Frankenberger, Jr.
University of California, Riverside campus
William T. Frankenberger is PI on this project
Dr. William T. Frankenberger
Environmental Science, Riverside campus
Dr. Frankenberger will share with 4 other PIs from California in the award distribution.
Microbial reduction of perchlorate to environmentally-acceptable innocuous end products is currently an area of intense interest. Although various microorganisms have been shown to reduce perchlorate and chlorate, to either chlorite or completely to chloride and molecular oxygen, there is limited biochemical and molecular data on the enzyme systems involved in perchlorate reduction. Such data would provide important information for the design and application of an efficient biological-based ground water remediation strategy for perchlorate. This project will evaluate perchlorate reduction by cell-free extracts of Perclace in aqueous batch experiments. Data achieved from thie experiments would be useful for field-scale studies of groundwater treatment. The use of a cell-free enzyme system for perchlorate reduction would help avert the need for a post-reactor to destroy biomass in the effluent.