Water Resources--Office of Water Quality

National Field Manual

Complete document in pdf format: Chapter7.1.pdf 325K
Separate sections are also available as pdf files.

7.1 FECAL INDICATOR BACTERIA

7.1.1: Equipment and equipment sterilization procedures
7.1.2: Sample collection, preservation, and storage; 7.1.2.A Surface-water sample collection
7.1.2.B Ground-water sample collection
7.1.2.C Sample preservation and storage
7.1.3: Identification and enumeration methods; 7.1.3.A Preparation of media and reagents
7.1.3.B Preparation, holding times, and specifications for culture media
7.1.3.C Membrane filtration procedure
7.1.4: Calculation and reporting of fecal indicator bacteria
7.1.5: Instructions for media preparation

Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Illustrations
7.1-1.	Diagram showing apparatus for obtaining a sample from a shallow well using sterile technique
7.1-2a. 7.1-2b	Photographs showing steps in membrane- filtration procedure
7.1-3.	Diagram showing preparation of sample volumes by dilution
7.1-4.	Diagram showing a method for counting colonies on gridded membrane filters
Tables
7.1-1.	Ranges of fecal indicator bacteria typically found in surface water and contaminated surface water
7.1-2.	Equipment and supplies used for membrane filtration analysis
7.1-3.	Equipment cleaning and sterilizing procedures
7.1-4.	Summary of equipment for sample collection and procedures for sample preservation of indicator bacteria
7.1-5.	Incubation times and temperatures for fecal indicator tests
7.1-6.	Recommended sample volumes for membrane filtration analyses based on ideal colony count and concentration range
7.1-7.	Membrane-filter pore sizes for fecal indicator bacteria tests
7.1-8.	Preparation guidelines for dilution of samples to volumes less than 1.0 milliliter for fecal indicator bacteria analysis
7.1-9.	Test (media type), ideal colony count, and typical colony color, size, and morphology for indicator bacteria colonies

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Last Modified: 1FEB99 imc